Composite
CNS1-CNS2

Part:BBa_K3221103:Design

Designed by: Liu Yuxi   Group: iGEM19_SCU-China   (2019-09-30)


GAL1 and T7 promoter, coding sequence of CNS1 and CNS2 with 6×His tag and CYC terminator.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4819
    Illegal SpeI site found at 1266
    Illegal SpeI site found at 1795
    Illegal SpeI site found at 2449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1266
    Illegal SpeI site found at 1795
    Illegal SpeI site found at 2449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1843
    Illegal BglII site found at 2430
    Illegal BglII site found at 2848
    Illegal BamHI site found at 2932
    Illegal XhoI site found at 4813
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4819
    Illegal SpeI site found at 1266
    Illegal SpeI site found at 1795
    Illegal SpeI site found at 2449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4819
    Illegal SpeI site found at 1266
    Illegal SpeI site found at 1795
    Illegal SpeI site found at 2449
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1909
    Illegal AgeI site found at 3372
    Illegal AgeI site found at 4882
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did codon optimization of Saccharomyces Cerevisiae to this part.

Source

The original sequence of CNS1 and CNS2 comes from NCBI. The samples used for experiments are artificially synthesized by Sangong Biotech.

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.